3. a. The 5 subjects of experiments lasting 4-10 weeks were all rabbits, taking gradually increasing does of 1st trit. In about 2 weeks a thin watery discharge from nose came on, and gradually increased in quantity and consistency, till nostrils were partially obstructed, and hair beside them matted together. Soon after commencement of coryza fur of coat began to stick out and lose its smoothness; three layers of white scales formed on skin at different parts, and gradually spread over whole surface, but were thicker at neck, back, and thighs. Animals lost flesh rapidly, even to emaciation, before appetite and excretions were perceptibly affected. They then got dull and weak, and trembled in moving about; refused food and drink, and finally died.
3 b. P. M. – Parts of skin corresponding to scurfy patches were thickened, and corium of cartilaginous consistence, when cut into white and shining like fibro-cartilaginous consistence, when cut into white and shining like fibro- cartilage. Muscles of body thin and wasted. Mucous membrane of stomach thickened, pulpy, easily stripped off; outer surface of viscus red. In No. 1 small intestine reddened internally, in Nos.3 and 4 outwardly only; but in all containing viscid gelatinous mucus. In No.. 1 considerable injection of large intestine at point of junction with small, and coats of gut had lost their transparency; in No.3 and 4 outwardly only; but in all containing viscid gelatinous mucus. In No. 1 considerable injection of large intestine at point of injunction with small and coats of gut had lost their transparency; in No.3 coats easily torn; in No.5 between muscular and peritoneal coats of whole colon a gelatinous mass; in No.2 rectum constricted in 3 places. Contents were pitch, green and clayey faeces. Mesenteric vessels were gorged and glands enlarged. Liver in every case enlarged, in some even to twice its natural size; full of blood and friable, easily rubbed to pulp between fingers; colour dark reddish brown or even blackish. Gall – bladder full of bile in all cases.
3 c. In No. 1 tubular substance of kidneys somewhat softer than natural, and reddened; bladder full of clear watery urine. In No. 2 kidneys dense and firm, cortical and tubular parts not to be distinguished by their colour, which was in both dark brownish red; bladder quite filled with thickish yellow purulent – looking urine, its coats soft but without trace of inflammation. In No. 3 kidneys outwardly dark coloured, and inwardly showed only slight traces of tubular substance. In Nos. 4 and 5, kidneys when cut into showed brownish – red colour, and no trace of tubular substance. In 3 last cases bladder was quite empty.
3 d. In No. 5 nasal passages were reddened. In No. 2 they contained cheesy white soft masses; m. m. was softened and discoloured showing several spots like small ulcers. In Nos. 1, 2, and 4 lungs were collapsed, so as in 1 only to fill posterior cavity of chest; colour deep pink or cinnabar; unaltered in tissue, crepitating and swimming in water. In No. 3 dependent part of both lungs showed marks of inflammation, and sank somewhat in water.
3 e. In all cases auricles and ventricles filled with dark, clotted blood. In No. 3 heart was enlarged to nearly size of a lung; in No.4 to that of both lungs together, pericardium reddened, and coronary arteries full of blood.
3 f. In No. 4 meningeal vessels and sinuses gorged with blood; surface of left hemisphere very vascular. In No. 3 membranes were reddened; and posteriorly there was extravasation of dark blood on surface of brain. In No.2 membranes, somewhat reddened, and considerable vascular development in ventricles; brain very soft. In No.1 vascular development in membranes, which were uniformly reddened; vessels of cerebellum and medulla oblongata as if injected substance of brain very soft, and falling into pulp under pressure of finger. (Ibid.
Hughes was a great writer and a scholar. He actively cooperated with Dr. T.F. Allen to compile his 'Encyclopedia' and rendered immeasurable aid to Dr. Dudgeon in translating Hahnemann's 'Materia Medica Pura' into English. In 1889 he was appointed an Editor of the 'British Homoeopathic Journal' and continued in that capacity until his demise. In 1876, Dr. Hughes was appointed as the Permanent Secretary of the Organization of the International Congress of Homoeopathy Physicians in Philadelphia. He also presided over the International Congress in London.