2. 10 gr. were dissolved in z 33 of water containing 3j of hydriodate of potash, and the whole of the fluid was administered to a large, young Newfoundland dog. The animal manifested strong dislike to the taste and in 4 or 5 m. vomited a quantity of frothy mucus, with violent retching, which was repeated about four times within the hours, the later matter brought up exhibiting streaks of blood. During this time great uneasiness was indicated by constant changes from the standing to the lying posture, and the reverse; and after several attempts to relieve the bowels, the animal succeeded in about 1/2 hours in evacuating a loose stool. The whole of the following day was spent in a state of marked depression, after which he began to take nourishment, and in 4 or 5 day seemed to be not at all under the influence of the poison. (Ibid.).
Hughes was a great writer and a scholar. He actively cooperated with Dr. T.F. Allen to compile his 'Encyclopedia' and rendered immeasurable aid to Dr. Dudgeon in translating Hahnemann's 'Materia Medica Pura' into English. In 1889 he was appointed an Editor of the 'British Homoeopathic Journal' and continued in that capacity until his demise. In 1876, Dr. Hughes was appointed as the Permanent Secretary of the Organization of the International Congress of Homoeopathy Physicians in Philadelphia. He also presided over the International Congress in London.